The mouse that we clone will be brown.
Materials:
Mimi- Mouse we will clone (Brown)
Megdo- Egg cell donor (Black)
Momi- Surrgate mother to grow Mimi clone (White)
Microscope
Petri Dishes
Sharp Pipette
Blunt Pipette
Chemical to Stimulate cell division
Procedures:
- Isolate donors cells from mimi and megdo
- Remove and discard the nucleus from the egg cell
- Transfer the somatic cell nucleus into the enucleated egg cell
- Stimulate cell division
- Implant the embryo into Momi the surrogate mother
- Deliver the baby mouse clone of Mimi
Results:
By using the egg cell and the Somatic cells I was able to clone Mimi. First I had to suck the nucleus form the egg cell which made it an enucleated egg cell. Then put it in a dish. Then I had to move the somatic cell with the enucleated egg. Under the microscope I had to suck the Nucleus form the Somatic cell and inject in into the enucleated egg cell. In order for the DNA to re-programmed it takes several hours. Then I had to put a drop of a liquid chemical that mimics the cellular events. Then we have to wait several hours for the cell to divide a few times. After all thats done we have to insert the embryo into the womb of Momi. After 19 days we are ready to deliver to baby mouse.
Conclusion:
In conclusion the mouse came out exactly the same as Mimi. In my hypothesis I stated the the mouse we clone will be brown. I was right. All the genetics came from the somatic nucleus donor.
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